Process for the production of alpha-human atrial natriuretic polypeptide
AssigneeFujisawa Pharmaceutical Co., Ltd.
InventorsIkuo Ueda, Mineo Niwa, Yoshimasa Saito, Hisashi Yamada, Yoshinori Ishii
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Abstract
The present invention relates to a process for the production of α-human atrial natriuretic polypeptide by recombinant DNA technology.
Claims19
A process for the production of α-hANP by (1) culturing a microorganism transformed with an expression vector comprising a synthetic gene encoding an amino acid sequence of a protective peptide-fused α-hANP in a nutrient medium, (2) recovering the protective peptide-fused α-hANP from the cultured broth and (3) removing the protective peptide portion of the protective peptide-fused α-hANP.
A chemically synthsized gene encoding amino acid sequence of α-hANP:
- H-Ser-Leu-Arg-Arg-Ser-Ser-Cys-Phe-Gly-Gly-Arg-Met-Asp-Arg-Ile-Gly-Ala-Gln-Ser-Gly-Leu-Gly-Cys-Asn-Ser-Phe-Arg-Tyr-OH
A chemically synthesized protective peptide gene having DNA sequence encoding lysine as C-terminal of the protective peptide.
A recombinant vector comprising chemically synthesized α-hANP gene.
A recombinant vector of claim 13, in which the α-hANP gene is represented by the following DNA sequence:
- 8 Coding:
- 5′-TCT CTG CGT AGA TCC TCT TGC TTT GGT Noncoding:
- 3′-AGA GAC GCA TCT AGG AGA ACG AAA CCA GGC CGT ATG GAC CGC ATC GGT GCT CAG TCC GGT CTG CCG GCA TAC CTG GCG TAG CCA CGA GTC AGG CCA GAC GGC TGT AAC TCT TTC CGT TAC-3′ CCG ACA TTG AGA AAG GCA ATG-5′
A recombinant vector comprising chemically synthesized gene represented by the DNA sequence of FIG. 17.
A recombinant vector comprising chemically synthesized gene represented by the DNA sequence of FIG. 4.
A transformant comprising expression vector of chemically synthesized α-hANP gene.
A cleaving method of the fused-protein composed of a peptides having a lysine between a protective peptide and a targent peptide in the presence of API.
Synthetic trp promoter III represented by the DNA sequence of FIG. 3.
Dependencies are inferred from the claim text.