Gene family encoding membrane steroid receptors
OwnerYong Zhu, Peter Thomasinventors
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Abstract
A family of genes that encode membrane steroid receptors is identified in mammals and fish. A putative progestin membrane receptor (mPR) from a fish ovary was cloned, expressed and characterized. Database searching with the fish ovary mPR lead to the discovery of a new family of steroid membrane receptor genes with highly conserved sequences and structures similar to fish mPR in human, mouse and swine tissues. The cDNA sequence for each of these mammalian genes was determined. The cDNAs appear to be members of a novel gene family, not closely related to any previously characterized vertebrate genes.
Claims39
An isolated nucleic acid sequence, and complements and homologues thereof, comprising a sequence that hybridizes under stringent conditions to a hybridization probe, wherein the nucleotide sequence of the hybridization probe comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9, SEQ ID NO:
- 11, SEQ ID NO:
- 13 and SEQ ID NO:
- 15.
A trans-species family of genes encoding for a membrane steroid receptor, wherein each gene of the family comprises or has homology to a spotted seatrout (Cynoscion nebulosus) gene that encodes for an ovarian membrane progestin (20β-S) receptor.
A purified membrane steroid receptor.
A membrane steroid receptor capture protocol comprising the steps of:
- forming a complex between solubilized membrane proteins and an antibody and an antigen that specifically binds to the antibody;
- exposing a steroid to the complex, whereby the steroid specifically binds to the complex;
- and
- identifying a receptor to which the steroid is specifically bound.
Dependencies are inferred from the claim text.