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US 2003/0170781 A1Published application
Secreted protein zacrp4
AssigneeZymoGenetics, Inc.
InventorsJames L. Holloway, Si Lok
Life of this patent
Estimating the term…
Abstract
The present invention relates to polynucleotide and polypeptide molecules for zacrp4, a secreted protein having tandem C1q globular domains. Zacrp4 is highly expressed in neuronal and reproductive tissues and may be used in the study cell-cell communication and the regulation of cellular processes therein. The present invention also includes antibodies to the zacrp4 polypeptides.
Claims43
An isolated polypeptide comprising:
- a first C1q domain comprising a sequence of SEQ ID NO:3, 10 beta strands corresponding to amino acid residues 31-35, 52-54, 60-63, 67-69, 73-84, 89-95, 101-108, 112-126, 131-136 and 150-154 of SEQ ID NO:2, and a cysteine residue corresponding to residue 70 of SEQ ID NO:2;
- and
- a second C1q domain joined to the carboxy terminal of said first C1q domain, said second C1q domain comprising a sequence of SEQ ID NO:3, 10 beta strands corresponding to amino acid residues 179-183, 206-208, 214-217, 221-223, 227-238, 243-249, 254-262, 267-278, 283-288 and 305-309 of SEQ ID NO:2, and a cysteine residue corresponding to residue 223, a glycine residue corresponding to residue 228 of SEQ ID NO:2.
An isolated polypeptide comprising a sequence of amino acid residues that is at least 80% identical in amino acid sequence to residues 17 to 329 of SEQ ID NO:2, wherein said sequence comprises:
- a first C1q domain comprising a sequence of SEQ ID NO:3, 10 beta strands corresponding to amino acid residues 31-35, 52-54, 60-63, 67-69, 73-84, 89-95, 101-108, 112-126, 131-136 and 150-154 of SEQ ID NO:2, and a cysteine residue corresponding to residue 70 of SEQ ID NO:2;
- and
- a second C1q domain joined to the carboxy terminal of said first C1q domain, said second C1q domain comprising a sequence of SEQ ID NO:3, 10 beta strands corresponding to amino acid residues 179-183, 206-208, 214-217, 221-223, 227-238, 243-249, 254-262, 267-278, 283-288 and 305-309 of SEQ ID NO:2, and a cysteine residue corresponding to residue 223, a glycine residue corresponding to residue 228 of SEQ ID NO:2.
An isolated polypeptide comprising:
- a signal sequence;
- a first C1q domain comprising a sequence of SEQ ID NO:3, 10 beta strands corresponding to amino acid residues 31-35, 52-54, 60-63, 67-69, 73-84, 89-95, 101-108, 112-126, 131-136 and 150-154 of SEQ ID NO:2 and a cysteine residue corresponding to amino acid residue 70 of SEQ ID NO:2;
- and
- a second C1q domain joined to the carboxy terminal of said first C1q domain, said second C1q domain comprising a sequence of SEQ ID NO:3, 10 beta strands corresponding to amino acid residues 179-183, 206-208, 214-217, 221-223, 227-238, 243-249, 254-262, 267-278, 283-288 and 305-309 of SEQ ID NO:2 and a cysteine residue corresponding to residue 223 of SEQ ID NO:2;
- wherein said polypeptide specifically binds with an antibody that specifically binds with a polypeptide consisting of the amino acid sequence of SEQ ID NO:2.
2 more elements
An isolated polypeptide selected from the group consisting of:
- a) a polypeptide consisting of amino acid residue 17 to amino acid residue 159 of SEQ ID NO:2 or
- b) a polypeptide consisting of amino acid residue 160 to amino acid residue 329 of SEQ ID NO:2.
An isolated polynucleotide molecule encoding a polypeptide comprising:
- a signal sequence;
- a first carboxyl-terminal C1q domain comprising a sequence of SEQ ID NO:3, 10 beta strands corresponding to amino acid residues 31-35, 52-54, 60-63, 67-69, 73-84, 89-95, 101-108, 112-126, 131-136 and 150-154 of SEQ ID NO:2 and a cysteine residue corresponding to amino acid residue 70 of SEQ ID NO:2;
- and
- a second carboxyl-terminal C1q domain comprising a sequence of SEQ ID NO:3, 10 beta strands corresponding to amino acid residues 179-183, 206-208, 214-217, 221-223, 227-238, 243-249, 254-262, 267-278, 283-288 and 305-309 of SEQ ID NO:2 and a cysteine residue corresponding to residue 223 of SEQ ID NO:2;
- wherein said polypeptide specifically binds with an antibody that specifically binds with a polypeptide consisting of the amino acid sequence of SEQ ID NO:2, and
- said polynucleotide molecule remains hybridized following stringent wash conditions to a polynucleotide consisting of the nucleotide sequence of SEQ ID NO:
- 1, or the complement of SEQ ID NO:1.
4 more elements
An isolated polynucleotide molecule selected from the group consisting of,
- a) a contiguous sequence of nucleotides from nucleotide 1 to nucleotide 1357 of SEQ ID NO:1;
- b) a contiguous sequence of nucleotides from nucleotide 210 to nucleotide 1196 of SEQ ID NO:1;
- c) a contiguous sequence of nucleotides from nucleotide 258 to nucleotide 1196 of SEQ ID NO:1;
- d) a contiguous sequence of nucleotides from nucleotide 258 to nucleotide 686 of SEQ ID NO:1;
- e) a polynucleotide encoding a polypeptide consisting of the sequence of amino acid residues 17 to 159 of SEQ ID NO:2;
- f) a polynucleotide encoding a polypeptide consisting of the sequence of amino acid residues 160 to 329 of SEQ ID NO:2;
- g) a polynucleotide encoding a polypeptide consisting of the sequence of amino acid residues 17 to 329 of SEQ ID NO:2;
- h) a polynucleotide that remains hybridized following stringent wash conditions to a polynucleotide consisting of the nucleotide sequence of SEQ ID NO:1, or the complement of SEQ ID NO:
- 1;
- i) the nucleotide sequences complementary to a), b), c), d), e), f), g) or h) and m) degenerate nucleotide sequences of e), f) or g).
7 more elements
An isolated polynucleotide molecule consisting of the sequence of nucleotide 1 to nucleotide 987 of SEQ ID NO:4.
Dependencies are inferred from the claim text.