US 2003/0170773 A1 · Nutritional mixture and method for early identification and count of gram-negative organisms

Abstract

The present invention is related with Microbiology and particularly with a nutrient mixture and a procedure for the identification and the early count of Gram-negative organisms. This mixture allows the development of five different colony colors in the organisms to be detected, and the appearance of three colored fluorescent emissions, the appearance of three different colors halos and zones with opaque precipitates surrounding such colonies. Those factors, together with the color changes in the medium, allow a differentiation with a high sensibility and specificity level. The mixture include specific relations of the mixtures of tryptophan rich protein fractions, free tryptophan, organic or inorganic salts, color or fluorescence providing substances, cellulose and hemi cellulose and other components which provide from 1 to 2 layers in the composition.

Claims13

Dependent claims

A nutrient mixture for the identification and early counting of Gram-negative organisms wherein said mixture contains:

a mixture of tryptophan rich protein fractions or free tryptophan, which is in quantities from 22 to 46% of the total mixture (weight/weight);
a mixture of selected organic and/or inorganic salts in quantities from 15 to 20% of the total mixture (weight/weight);
a mixture of substances providing color or fluorescence in quantities from 0,3 to 37% of the total mixture (weight/weight);
inhibitor substances of the Gram-positive organisms in quantities from 2 to 4,5% of the total mixture (weight/weight);
and
a mixture of substances providing solid structure to the medium, in quantities from 15 to 50%.

3 more elements

A procedure for the identification and early count of Gram negative organisms, wherein the nutritive mixture of any of the claims from 1 to 9, after its solidification and being in contact with the microorganisms or samples containing them, it is incubated for a period from 12 to 22 hours, at a temperature from 30 to 45° C., from which it is possible the identification of said microorganisms at first sight, while fluorescence detection is carried out under ultraviolet light from 360 to 366 nm.

Dependencies are inferred from the claim text.

Nutritional mixture and method for early identification and count of gram-negative organisms

Life of this patent

Nutritional mixture and method for early identification and count of gram-negative organisms

Life of this patent

Abstract

Claims13