Rapid, immunochemical process for measuring thiopurine methyltransferase
AssigneeBiologix Diagnostics, LLC
InventorsNisha V. Padhye, Andre?apos; Quintanar, R. Michael Nelson
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Abstract
The present invention relates to non-isotopic immunoassays for thiopurine methyltransferase (TPMT). The immunoassays of this invention may be homogenous or heterogenous, in which detection of the TPMT-catalyzed reaction product relies upon specific binding of antibody to 6-MMP or other TPMT-catalyzed reaction products. Preferred embodiments of this invention include a Rapid Immunomigration Cassette and an assay carried out in ELISA assay format.
Claims25
A method of detecting thiopurine methyltransferase (TPMT) comprising:
- obtaining a biological sample from an individual;
- and assaying for the level of a TPMT-catalyzed reaction product.
A rapid immunomigration cassette comprising:
- a) an immunocassette;
- b) chromatography paper, said chromatography paper having antibody specific to a methylated reaction product of TPMT and additional capture molecule dried thereon, said antibody being affixed to gold molecules, said chromatography paper being encased in the immunocassette;
- c) a biological sample suspected of containing methylated reaction product of TPMT.
A kit for quantitatively measuring the levels of TPMT in a biological sample comprising:
- a) a biological sample suspected of containing TPMT;
- b) an ELISA plate, said ELISA plate being coated with a carrier protein-6-MP conjugate;
- c) antibody specific to a methylated reaction product of TPMT;
- d) means for labeling the conjugate that is methylated by the TPMT;
- and e) means for measuring the optical signal of the methylated conjugate.
Dependencies are inferred from the claim text.