Ragweed allergens
OwnerBob B. Buchanan, Gregorio del Val +1inventors
InventorsBob B. Buchanan, Gregorio del Val, Oscar L. Frick
Life of this patent
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Abstract
A 30 kDa ragweed complete pollen extract disulfide protein allergen has been purified from ragweed pollen. IgE immunoblots with sera of ragweed, walnut and ryegrass sensitive patients indicated that the 30 kDa protein is a major allergen. The 30 kDa protein finds use in allergy testing and immunotherapy regimens.
Claims46
An isolated protein comprising an amino acid sequence wherein said amino acid sequence is selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, and SEQ ID NO:
- 11.
An isolated nucleic acid compromising a nucleotide sequence encoding an amino acid sequence wherein said amino acid sequence is selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, and SEQ ID NO:
- 11.
An isolated pollen allergen substantially free of any other pollen proteins characterized by the following physiochemical and biological properties:
- a) being contained in pollen extracts, b) a glycoprotein, c) a sulfhydryl group containing protein, d) a molecular weight about 30,000 as determined by SDS-polyacrylamide gel electrophoresis and e) possessing allergen activity.
A therapeutic composition comprising an antigenic fragment of a ragweed pollen allergen Ambt 7 wherein said antigenic fragment comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, and SEQ ID NO:
- 11 wherein said antigenic fragment comprises at least one epitope of said pollen allergen and a pharmaceutically effective carrier.
A therapeutic composition comprising an Ambt7 pollen allergen which is a polymorphic variant of a ragweed Ambt7 pollen allergen wherein said polymorphic variant comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, and SEQ ID NO:
- 11 and a pharmaceutically acceptable carrier.
A kit for detecting Ambt7 pollen allergen comprising one or more proteins wherein said one or more proteins comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 61 SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, and SEQ ID NO:
- 11.
A method of purifying a pollen allergen, comprising:
- a) suspending said pollen in a liquid to form a pollen solution;
- b) centrifuging said pollen solution to produce a pollen protein supernatant;
- c) precipitating said protein in said pollen protein supernatant to form a protein precipitate;
- d) resuspending said protein precipitate in a protein precipitate buffer to form a resuspended protein mixture;
- e) extracting said resuspended protein mixture in organic solvent to form an aqueous phase and an organic phase;
- and
- f) purifying said pollen allergen from said aqueous phase.
An isolated antibody that binds specifically to a protein comprising an amino acid sequence wherein said amino acid sequence is selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, and SEQ ID NO:
- 11.
An isolated antibody that binds specifically to a pollen allergen substantially free of any other pollen proteins wherein said pollen allergen is characterized by the following physiochemical and biological properties:
- a) being contained in pollen extracts, b) a glycoprotein, c) a sulfhydryl group containing protein, d) a molecular weight about 30,000 as determined by SDS-polyacrylamide gel electrophoresis and e) possessing allergen activity.
Dependencies are inferred from the claim text.