Back to results
US 2003/0170760 A1Published application
Cell based signal generation
AssigneeCadus Pharmaceutical Corporation
InventorsJun Xu, Joshua Trueheart
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeCadus Pharmaceutical Corporation
InventorsJun Xu, Joshua Trueheart
Estimating the term…
The present invention makes available a rapid, reproducible, robust assay system for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular protein, e.g., a receptor or ion channel. The subject assay enables rapid screening of large numbers of compounds to identify those which act as an agonist or antagonist to the bioactivity of the cellular protein. In particular, the assay of the invention makes use of a cell that harbors a protein that is responsive to a cellular signal transduction pathway. The protein is operatively linked to a polypeptide which causes a detectable signal to be generated upon stimulation of the pathway, e.g., when a compound interacts with and modulates the activity of a cellular receptor or ion channel of the cell. Thus, the cell provides a signal generation means comprising a novel fusion protein the expression of which is independent of stimulation/activation of the signal transduction pathway, but the activity of which is responsive to the signal transduction pathway.
A recombinant yeast cell comprising a yeast protein operatively linked to a polypeptide, wherein said yeast protein is responsive to a signal transduction pathway of the cell, and the activity of said yeast protein is modulated directly or indirectly upon stimulation of said pathway, and wherein upon modulation of said yeast protein, said polypeptide causes a detectable signal to be generated.
A recombinant yeast cell comprising a yeast protein operatively linked to a chimeric transcription factor, and a gene which produces a detectable protein, wherein said yeast protein is responsive to a yeast pheromone response pathway of the cell, and wherein the activity of said yeast protein is modulated directly or indirectly upon stimulation of said pathway, and wherein upon modulation of said yeast protein, said chimeric transcription factor causes a detectable signal to be generated.
A method for identifying a test compound that modulates a heterologous receptor expressed by a recombinant yeast cell comprising:
A chimeric nucleic acid construct comprising:
Dependencies are inferred from the claim text.