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US 2003/0170751 A1Published application
Immunohistochemical assay for ras activity
OwnerLawrence S. Sherman, Nancy Ratnerinventors
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Abstract
The present invention generally relates to methods for diagnosis and prognosis of cancers and more particularly to methods of detecting activated ras oncogene in cells and tissues. This method makes use of a fusion protein construct comprising the ras oncogene binding domain (“RBD”) of the raf protein which is linked to GST. This forms a “raf-GST” construct that binds to the ras oncogene only when ras is activated, i.e., in the ras-GTP state.
Claims6
A method for identifying activated Ras oncogene, comprising:
- a. incubating a biological sample with a buffer that prevents nonspecific binding of an epitope tag
- b. incubating a biological sample with Ras-binding peptide having an amino acid sequence with 80-100% sequence identity to a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and
- c. detecting the conjugated Ras-binding peptide
- wherein the incubations are carried out under conditions sufficient to allow the components to interact.
1 more element
Dependencies are inferred from the claim text.