Loading patent…
OwnerRobert B. Grieve, Glenn R. Frank +3inventors
InventorsRobert B. Grieve, Glenn R. Frank, Roy R. Mondesire, James P. Porter, Nancy Wisnewski
Estimating the term…
The present invention includes a method to detect D. immitis infection in a host animal using a D. immitis Di33 protein to detect anti-D. immitis Di33 antibodies in a bodily fluid of the animal. Also included is a method to detect D. immitis infection in a host animal using a D. immitis anti-Di33 protein to detect Di33 proteins in a bodily fluid of the animal. The present invention also relates to D. immitis detection kits that include either a Di33 protein or an anti-Di33 antibody; such kits also include a composition to detect an immunocomplex between the anti-Di33 antibody and D. immitis Di33 protein. The present invention also includes Di33 proteins, nucleic acid molecules encoding such proteins, as well as recombinant molecules and recombinant cells comprising such nucleic acid molecules, and anti-Di33 antibodies. Also included are methods to produce such proteins, nucleic acid molecules and antibodies.
A method to detect D. immitis in a non-adapted host comprising:
A method to detect D. immitis in a host animal, said method comprising:
A method to detect D. immitis infection in a non-adapted host within 10 weeks of infection, said method comprising detecting anti-Di33 antibodies in a bodily fluid collected from said host.
A kit to detect D. immitis infection comprising an isolated D. immitis Di33 protein and a composition to detect antibodies capable of forming an immunocomplex with said Di33 protein.
A method to produce an isolated D. immitis Di33 protein comprising:
A method for purifying a D. immitis Di33 protein comprising recovering Di33 protein from cation exchange chromatography of disrupted insoluble material obtained from a culture of D. immitis Di33 protein-producing recombinant cells.
A protein selected from the group consisting of PHIS-PDi33234 and PDi33217.
A nucleic acid molecule selected from the group consisting of nDi33346, nDi33750, nDi33702, nDi33708, and nDi33651.
A method to detect D. immitis in a non-adapted host comprising:
A kit to detect D. immitis infection comprising an isolated anti-Di33 antibody and a composition to detect an immunocomplex between said anti-Di33 antibody and D. immitis Di33 protein.
Dependencies are inferred from the claim text.