Compositions and methods for increasing amino acid absorption in mammals
AssigneeThe Iams Company
InventorsGary Mitchell Davenport, Jamie Clyde Matthews
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Abstract
The present invention provides novel isolated and purified nucleic acid (RNA or DNA) encoding, or complementary to, a canine PepT1 (cPepT1). The present invention also provide a method for determining canine PepT1-transportability of a peptide, or method for determining a peptide with beneficial nutritional property in an animal. The present invention further provides a dietary composition for an animal comprising a peptide identified by the method described above.
Claims26
A method for determining canine PepT1 -transportability of a peptide comprising:
- (a) providing an immortalized kidney distal tubule epithelial (Madin-Darby Canine Kidney (MDCK)) cell and a peptide having about 2 to about 4 amino acids, and
- (b) determining the amount of the peptide transported into the cell, wherein the amount correlates with the canine PepT1-transportability of the peptide.
A method for identifying a peptide with a beneficial nutritional property for an animal comprising:
- (a) providing an immortalized kidney distal tubule epithelial (Madin-Darby Canine Kidney (MDCK)) cell and a peptide having about 2 to about 4 amino acids, and
- (b) determining the amount of the peptide transported into the cell, wherein the amount correlates with the beneficial nutritional property.
A method of stimulating H+-dependent peptide transport in cells comprising contacting the cells (in vitro or in vivo) with a PepT1 substrate.
A composition comprising an isolated nucleic acid encoding, or complementary to, a canine PepT1.
A peptide having an amino acid sequence encoded by the nucleic acid of SEQ ID NO:7-9 or 20.
A peptide having an amino acid sequence encoded by SEQ ID NO:
- 13 or SEQ ID NO:21.
Dependencies are inferred from the claim text.