Polynucleotides and polypeptides associated with the development of osteoarthritis
OwnerJulie Carman, Steven G. Nadler +3inventors
InventorsJulie Carman, Steven G. Nadler, Michael Bowen, Michael G. Neubauer, Pin Lu
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Abstract
The present invention is directed to polynucleotides encoding polypeptides associated with the development of osteoarthritis and homologs thereof. The invention further relates to diagnostic and therapeutic methods for utilizing these polynucleotides and polypeptides in the diagnosis, treatment, and/or prevention of osteoarthritis and related disease states. The invention further relates to screening methods for identifying agonists and antagonists of the polynucleotides and polypeptides of the present invention, and compounds identified thereby.
Claims25
An assay for identifying a compound that modulates the activity of a gene associated with osteoarthritis, comprising:
- (1) providing a cell expressing a gene associated with osteoarthritis, wherein the nucleic acid sequence of said gene associated with osteoarthritis is at least 95% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, and SEQ ID NO:35;
- (2) contacting said cell expressing said gene associated with osteoarthritis with a test compound;
- and
- (3) determining whether said test compound modulates the activity of said gene associated with osteoarthritis.
An assay for identifying a compound that modulates the activity of a protein associated with osteoarthritis, comprising:
- (1) providing a cell expressing a gene associated with osteoarthritis, wherein said gene encodes a polypeptide having an amino acid sequence which is at least 95% identical to an amino acid sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, and SEQ ID NO:36;
- (2) contacting said cell expressing said gene associated with osteoarthritis with a test compound;
- and
- (3) determining whether said test compound modulates the activity of said protein associated with osteoarthritis.
A method for the treatment of osteoarthritis, comprising:
- (1) identifying a patient suffering from osteoarthritis;
- and
- (2) administering to said patient a therapeutically effective amount of a modulator of a gene associated with osteoarthritis,
- wherein said gene associated with osteoarthritis has a nucleic acid sequence which is at least 95% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, and SEQ ID NO:35.
A method for the treatment of osteoarthritis, comprising:
- (1) identifying a patient suffering from osteoarthritis;
- and
- (2) administering to said patient suffering from osteoarthritis a therapeutically effective amount of a modulator of a polypeptide associated with osteoarthritis,
- wherein said polypeptide associated with osteoarthritis has an amino acid sequence which is at least 95% identical to an amino acid sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, and SEQ ID NO:36.
A method for the prevention of osteoarthritis, comprising:
- (1) identifying a patient at risk for osteoarthritis;
- and
- (2) administering to said patient at risk for osteoarthritis a therapeutically effective amount of a modulator of a gene associated with osteoarthritis,
- wherein said gene associated with osteoarthritis has a nucleic acid sequence which is at least 95% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID
Dependencies are inferred from the claim text.