Characterized E2IG3 protein for diagnosis and treatment of proliferative diseases
OwnerChristos Hatzis, Nandan Padukone +1inventors
InventorsChristos Hatzis, Nandan Padukone, John G. Babish
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Abstract
The invention relates to the gene encoding the mammalian E2IG3 and its product. More specifically, the invention relates to the diagnosis of aberrant E2IG3 gene or gene product expression, the identification, production, and use of compounds which modulate E2IG3 expression or the activity of the E2IG3 gene product including but not limited to nucleic acid encoding E2IG3 and homologues, analogues, and deletions thereof, as well as antisense, ribozyme, triplehelix, antibody, and polypeptide molecules as well as small inorganic molecules; and pharmaceutical formulations and routes of administration for such compounds.
Claims18
A polypeptide comprising an E2IG3 sequence or a substantially identical fragment thereof.
A polynucleotide comprising a sequence encoding an E2IG3 polypeptide or substantially identical a fragment thereof.
A method for identification of E2IG3 polypeptide function comprising:
- treating cells with estrogen;
- determining polynucleotide expression patterns using a gene array;
- determining the polypeptide phosphorylation expression patterns;
- and
- correlating the polynucleotide and polypeptide phosphorylation expression patterns.
An antibody that binds to an E2IG3 polypeptide or a substantially identical fragment thereof.
A method for identification of molecules that modulate E2IG3 expression comprising:
- adding candidate molecules to the culture medium of cells expressing E2IG3 mRNA;
- measuring E2IG3 expresssion;
- and
- comparing the level of E2IG3 expression in the presence of the candidate molecules to the level of E2IG3 expression in the absence of the candidate molecules.
A method for identification of molecules that modulate E2IG3-mediated apoptosis comprising:
- adding candidate molecules to the culture medium of cells expressing E2IG3 mRNA;
- measuring the degree of apoptosis;
- and
- comparing the degree of apoptosis in the presence of the candidate molecules to the degree of apoptosis in the absence of the candidate molecules.
Dependencies are inferred from the claim text.