Methods of identifying target polypeptides

A method of rank ordering potential targets of a candidate polypeptide comprising:

• (a) generating a functional profile for a set of polypeptides comprising the candidate polypeptide and a set of mutants derived therefrom;
• (b) generating a plurality of interaction profiles for the set of polypeptides, wherein each interaction profile corresponds to a potential target for the candidate polypeptide;
• and
• (c) creating a rank order of the potential targets based upon the degree of correspondence between each interaction profile and functional profile, wherein the rank order of a potential target identifies the likelihood of interaction between the potential target and candidate polypeptide.

A method of rank ordering potential targets of a candidate polypeptide, comprising:

• (a) identifying a set of potential targets for the candidate polypeptide in an interaction assay;
• (b) generating a functional profile for a set of polypeptides comprising the candidate polypeptide and a set of mutants derived therefrom;
• (c) for each potential target identified in (a), generating an interaction profile for the set of polypeptides;
• (d) selecting those interaction profiles that correspond to the functional profile;
• and
• (e) creating a rank order of said potential targets based upon the degree of correspondence between each interaction profile and functional profile, wherein the rank order of a potential target identifies the likelihood of interaction between the potential target and candidate polypeptide.

A method of identifying a target of a candidate polypeptide comprising:

• (a) identifying a set of potential targets for the candidate polypeptide in an interaction assay;
• (b) generating a functional profile for a set of polypeptides comprising the candidate polypeptide and a set of mutants derived therefrom;
• (c) for each potential target identified in (a), generating an interaction profile for the set of polypeptides;
• (d) identifying an interaction profile that corresponds closely to the functional profile, thereby identifying the potential target that corresponds to the identified interaction profile as being a target of the candidate polypeptide.