Method of preparing a protein array based on biochemical protein-protein interaction
AssigneeThe Trustees of Columbia University in the City of New York
InventorTaka-Aki Sato
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Abstract
A method of preparing a protein array based on biochemical protein-protein interaction is provided. An array of a first protein which comprises a PDZ domain is deposited on a substrate. A second protein, which comprises an amino acid sequence (S/T)-X—(V/I/L)-COOH (each hyphen represents a peptide bond, each parenthesis encloses amino acids which are alternatives to one other, each slash within such parentheses separates the alternative amino acids, and the X represents any amino acid which is selected from the group comprising the twenty naturally occurring amino acids), is applied to the first protein array. The amino acid sequence (S/T)-X—(V/I/L)-COOH of the second protein is bound to the PDZ domain of the first protein.
Claims20
A method of preparing a protein array based on biochemical protein-protein interaction, comprising the steps of:
- (a) depositing on a substrate an array of a first protein, the first protein comprising a PDZ domain;
- and
- (b) applying a second protein, which comprises an amino acid sequence (S/T)-X—(V/I/L)-COOH, to the first protein array, the amino acid sequence (S/T)-X—(V/I/L)-COOH of the second protein binding to the PDZ domain of the first protein,
- wherein each hyphen represents a peptide bond, each parenthesis encloses amino acids which are alternatives to one other, each slash within such parentheses separates the alternative amino acids, and the X represents any amino acid which is selected from the group comprising the twenty naturally occurring amino acids.
A method of preparing a protein array, comprising the steps of:
- (a) depositing on a substrate an array of first proteins, each first protein comprising a corresponding PDZ domain;
- and
- (b) applying a second protein, which comprises an amino acid sequence (S/T)-X—(V/I/L)-COOH, to the array of first proteins, the amino acid sequence (S/T)-X—(V/I/L)-COOH of the second protein, for each of the first proteins, binding to the PDZ domain of the first protein,
- wherein each hyphen represents a peptide bond, each parenthesis encloses amino acids which are alternatives to one other, each slash within such parentheses separates the alternative amino acids, and the X represents any amino acid which is selected from the group comprising the twenty naturally occurring amino acids.
A method of preparing a protein array, comprising the steps of:
- (a) depositing on a substrate an array of a first protein, the first protein comprising a PDZ domain;
- and
- (b) applying a plurality of second proteins, each of which comprises a corresponding amino acid sequence (S/T)-X—(V/I/L)-COOH, to corresponding elements of the first protein array, for each of the second proteins, the amino acid sequence (S/T)-X—(V/I/L)-COOH of the second protein binding to the PDZ domain of the first protein in the corresponding array element,
- wherein each hyphen represents a peptide bond, each parenthesis encloses amino acids which are alternatives to one other, each slash within such parentheses separates the alternative amino acids, and the X represents any amino acid which is selected from the group comprising the twenty naturally occurring amino acids.
A method of preparing a protein array, comprising the steps of:
- (a) depositing on a substrate an array of a first polypeptide, the first polypeptide comprising a PDZ domain;
- and
- (b) applying a second polypeptide which comprises an amino acid sequence (S/T)-X—(V/I/L)-COOH to the first polypeptide array, the amino acid sequence (S/T)-X—(V/I/L)-COOH of the second polypeptide binding to the PDZ domain of the first polypeptide,
- wherein each hyphen represents a peptide bond, each parenthesis encloses amino acids which are alternatives to one other, each slash within such parentheses separates the alternative amino acids, and the X represents any amino acid which is selected from the group comprising the twenty naturally occurring amino acids.
Dependencies are inferred from the claim text.