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US 2003/0170715 A1Published application
Method for the rapid and ultra-sensitive detection of leukemic cells
AssigneeInvitrogen Corporation
InventorsPaul E. Nisson, Nicoletta Sacchi
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Abstract
An improved method is disclosed for diagnosing the presence of a chromosomal translocation characteristic of acute myelogenous leukemia. Nucleic acid molecules that may be used in this improved method are described.
Claims13
A method for diagnosing the presence of a leukemic cell in a sample comprising the steps:
- A) incubating said sample in the presence of at least one nucleic acid molecule that is capable of hybridizing to:
- (1) a nucleotide sequence found in chromosome 8 of a non-leukemic human cell or
- (2) a nucleotide sequence found in chromosome 21 of a non-leukemic human cell;
- and
- B) diagnosing said presence by determining whether said sample contains a nucleic acid molecule, or is capable of forming a single cDNA molecule, that is capable of hybridizing to both a nucleotide sequence found in chromosome 8 of a non-leukemic human cell and a nucleotide sequence found in chromosome 21 of a non-leukemic human cell.
2 more elements
A composition of matter comprising:
- (1) a nucleic acid molecule having a nucleotide sequence found in chromosome 8 of a non-leukemic human cell and
- (2) a nucleic acid molecule having a nucleotide sequence found in chromosome 21 of a non-leukemic human cell;
- wherein said nucleic acid molecules are capable of hybridizing to a chromosome at respective sites that flank a chromosome 8-chromosome 21 translocation characteristic of acute myelogenous leukemia.
Dependencies are inferred from the claim text.