Back to results
US 2003/0170711 A1Published application
Oligonucleotide probes and primers comprising universal bases for diagnostic purposes
AssigneeGen-Probe Incorporated
InventorsBob D. Brown, Timothy A. Riley
Life of this patent
Estimating the term…
Abstract
Aspects of the invention relate to novel oligonucleotides comprising universal and generic bases for use as primers and probes, as well as, methods of using said oligonucleotides for the diagnosis of disease.
Claims33
An oligonucleotide comprising between about 10 and 100 bases, wherein at least 2 of said bases are juxtaposed universal bases.
An oligonucleotide comprising the formula:
- XRY
- wherein X comprises between about 5 and 20 modified nucleic acid bases;
- wherein R comprises between about 3 and 10 juxtaposed universal bases;
- wherein Y comprises between about 5 and about 20 nucleic acid bases;
- wherein X, R, and Y are covalently joined;
- and
- wherein more than about 20% of the total number of bases in said oligonucleotide are said juxtaposed universal bases.
4 more elements
A method for detecting a polymorphism in a sample of genetic material, comprising:
- providing an oligonucleotide comprising between about 10 and 100 bases, wherein at least 2 of said bases are juxtaposed universal bases;
- contacting said genetic material with said oligonucleotide;
- and
- identifying whether said oligonucleotide is bound to said genetic material, wherein said universal or generic bases are not located at the site or sites of said polymorphism.
1 more element
A method for diagnosing a genetic disease in a patient, comprising:
- providing an oligonucleotide specific for a DNA sequence related to said disease, wherein said oligonucleotide comprises between about 10 and 100 bases, and wherein at least 2 of said bases are juxtaposed universal bases;
- contacting genetic material from said patient with said oligonucleotide;
- and
- identifying whether said oligonucleotide is bound to said genetic material, wherein binding of said oligonucleotide to said genetic material is indicative of said genetic disease.
1 more element
Dependencies are inferred from the claim text.