Method of separating viral particles
AssigneeAventis Pharma S.A.
InventorsFrancis Blanche, Anne Barbot, Beatrice Cameron
Life of this patent
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Abstract
The invention concerns a novel method for purifying and quantifying viral particles. More particularly, the invention concerns a method for purifying and quantifying adenovirus by ion-exchange chromatography. The invention also concerns a method for identifying different adenovirus serotypes.
Claims24
Method of separating viral particles from a biological medium, characterized in that it comprises at least one chromatography step carried out on a support comprising a matrix and ion-exchange groups, the said groups being grafted onto the said matrix by means of a flexible arm.
Use of a Q Sepharose® XL type chromatography support for the analytical and/or preparative separation of viral particles.
Use of a Q Sepharose® XL type chromatography support for the identification of various adenovirus serotypes.
Use of a Q Sepharose® XL type chromatography support for the titration of adenoviruses.
Method of quantifying adenoviruses, characterized in that the viral particles are separated by chromatography on a Q Sepharose® XL type support and the quantity of adenoviruses is measured by the absorbance of the chromatographic fractions.
Dependencies are inferred from the claim text.