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US 2003/0170706 A1Published application
Use of a volatile hybridization wash buffer
OwnerRoland Green, Michael Sussmaninventors
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Abstract
A method for washing and drying a surface that contains a salt and hybridized DNA or RNA molecules is disclosed. The method involves exposing the surface to a volatile buffer that does not denature a specific DNA/DNA, DNA/RNA or RNA/RNA hybridization on the surface, removing the volatile buffer, and evaporating residue volatile buffer.
Claims21
A method for washing a surface that contains a salt and hybridized DNA or RNA molecules, the method comprising the steps of:
- exposing the surface to a volatile buffer that does not denature a specific hybridization on the surface;
- removing the volatile buffer;
- and
- evaporating residue volatile buffer.
1 more element
A method for detecting DNA/DNA, DNA/RNA or RNA/RNA hybridization on a DNA or RNA microarray, comprising the steps of:
- providing a DNA or RNA microarray;
- incubating the microarray with a source of fluorescently labeled DNA or RNA in a hybridization buffer;
- removing the hybridization buffer;
- exposing the microarray to a volatile buffer that does not denature a specific hybridization on the microarray;
- removing the volatile buffer;
- evaporating residue volatile buffer;
- and
- scanning the microarray with a fluorescence scanner.
5 more elements
Dependencies are inferred from the claim text.