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US 2003/0170702 A1Published application
Methods for polysome distribution analysis of mRNA's
OwnerBryan Hoffmaninventor
Life of this patent
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Abstract
The invention provides methods for measuring the translation efficiency of an RNA of interest and methods for identifying a candidate compound that modulates the translation efficiency of the RNA.
Claims20
A method of amplifying an RNA molecule from a polysome distribution sucrose gradient, said method comprising the steps of:
- (a) purifying an RNA molecule in a solution from a polysome distribution sucrose gradient under conditions that reduce the amount of heparin in said solution by at least 90%;
- and
- (b) amplifying said purified RNA molecule.
A method of amplifying an RNA molecule that binds a compound of interest, said method comprising the steps of:
- (a) contacting one or more RNA molecules in a solution comprising heparin with a compound of interest under conditions that allow at least one RNA molecule to bind said compound of interest;
- (b) purifying said RNA molecule that binds said compound of interest under conditions that reduce the amount of heparin in said solution by at least 90%;
- and
- (c) amplifying said purified RNA molecule.
1 more element
Dependencies are inferred from the claim text.