Assay for identifying modulators of Borrelia telomere resolvase
OwnerGeorge Chaconas, Kerri Kobryn +1inventors
InventorsGeorge Chaconas, Kerri Kobryn, Yvonne M. Tourand
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Abstract
The present invention relates to a method of identifying modulators of Borrelia telomere resolvase. The method involves incubating the Borrelia telomere resolvase enzyme, ResT, in the presence of a test substance and a telomere resolution substrate comprising a functional replicated telomere. Substances identified using the method of the invention may be potent inhibitors of Borrelia infection.
Claims47
A method of identifying a modulator of ResT comprising:
- (a) incubating a test substance in the presence of ResT and a telomere resolution substrate;
- and
- (b) determining the effect of the test substance on telomere resolution, wherein a change in telomere resolution as compared to a control means the test substance is a modulator of ResT activity.
A method of modulating telomere resolution comprising administering an effective amount of a modulator of ResT to a cell or animal in need thereof.
A method of inhibiting;
- DNA replication comprising administering an effective amount of a modulator of ResT to a cell or animal in need thereof.
A method of treating or preventing Borrelia infection comprising administering an effective amount of an inhibitor of ResT to an animal in need thereof.
A method of treating or preventing a poxviral infection comprising administering an effective amount of an inhibitor of ResT to an animal in need thereof.
A kit for use in identifying a modulator of ResT comprising an aliquot of ResT and an aliquot of a telomere resolution substrate.
A method of preparing a pharmaceutical composition for use in modulating the activity of ResT comprising mixing a modulator of ResT with a suitable diluent or carrier.
An isolated ResT protein having the amino acid sequence shown in FIG. 2A (SEQ ID NO:
- 1) or an analog, homolog, isoform or fragment of the protein shown in FIG. 2A (SEQ ID NO:
- 1) that retains the telomere resolvase function.
Dependencies are inferred from the claim text.