Back to results
US 2003/0170684 A1Published application
Multiplexed methylation detection methods
AssigneeIllumina, Inc.
InventorJian-Bing Fan
Life of this patent
Estimating the term…
Abstract
The present invention is directed to sensitive and accurate multiplexed assays for target analyte detection and detection of methylation in nucleic acid samples.
Claims15
A method for multiplex detection of methylation of target nucleic acids comprising:
- a. providing a first population of target nucleic acids labeled with a purification tag;
- b. cleaving said first population of target nucleic acids with an enzyme, whereby said enzyme discriminately cleaves at methylated target sequences forming a second population of cleaved target sequences;
- c. immobilizing said first and second populations by said purification tag;
- and
- d. detecting the presence of said first population comprising non-cleaved target nucleic acid whereby the presence of said first population comprising non-cleaved target nucleic acid indicates the presence of methylated target nucleic acids.
2 more elements
A method of detecting methylation comprising:
- a. contacting a sample of target nucleic acids with bisulfite, whereby non-methylated cytosine is converted to uracil, forming a first population of treated target nucleic acids, and methylated cytosine is not converted to uracil, forming a second population of treated target nucleic acids;
- b. contacting said first and second populations of treated target nucleic acids with a first probe that hybridizes with a first target in said first population of target nucleic acid and a second probe that hybridizes with a target in said second population of target nucleic acid, forming first and second hybridization complexes, respectively;
- c. contacting said first and second hybridization complexes with an enzyme that modifies said first and second probes forming first and second modified probes;
- d. detecting said first and second modified probes to determine the presence of methylation in said target nucleic acid.
1 more element
Dependencies are inferred from the claim text.