Nucleic acid mediated electron transfer

A method of detecting a target sequence in a nucleic acid wherein said target sequence comprises a first target domain and a second target domain adjacent to said first target domain, wherein said method comprises:

• a) hybridizing a first nucleic acid containing at least one covalently attached electron donor moiety to said first target domain;
• b) hybridizing a second nucleic acid containing at least one covalently attached electron acceptor moiety to said second target domain, wherein at least one of said electron donor and acceptor moieties is covalently attached to a terminal base of the nucleic acid;
• and
• c) detecting electron transfer between said electron donor and said electron acceptor moieties.

A method of detecting a target sequence in a nucleic acid wherein said target sequence comprises a first target domain and a second target domain adjacent to said first target domain, wherein said method comprises:

• a) hybridizing a first nucleic acid containing at least one covalently attached electron donor moiety to said first target domain;
• b) hybridizing a second nucleic acid containing at least one covalently attached electron acceptor moiety to said second target domain, wherein at least one of said electron donor and acceptor moieties is an organic electron transfer moiety;
• and
• c) detecting electron transfer between said electron donor and said electron acceptor moieties.

A composition comprising:

• a) a first 2′-amino modified nucleoside covalently attached to a solid support;
• b) additional nucleosides covalently attached to the 5′ position of said first modified nucleoside, forming an oligonucleotide;
• and
• c) a second 2′-amino modified nucleoside incorporated into said oligonucleotide.

A method for making a nucleic acid with an electron transfer moiety attached at the 3′ terminus of said nucleic acid, said method comprising:

• a) attaching a 2′-amino modified nucleoside to a solid support;
• b) adding phosphoramidite nucleosides to the 5′ terminus of said modified nucleoside to form a nucleic acid;
• and
• c) adding an electron transfer moiety to said 2′-amino modified nucleoside.

A method for making a nucleic acid with an electron transfer moiety attached at the 5′ terminus of said nucleic acid, said method comprising:

• a) attaching a nucleoside to a solid support;
• b) adding phosphoramidite nucleosides to the 5′ terminus of said modified nucleoside to form a nucleic acid;
• c) adding a 2′ or 3′ amino modified nucleoside to the 5′ terminus of said nucleic acid;
• d) adding an electron transfer moiety to said 2′ or 3′-amino modified nucleoside.

A method for making a nucleic acid with electron transfer moieties attached at the 3′ and 5′ terminus of said nucleic acid, said method comprising:

• a) attaching a modified nucleoside to a solid support;
• b) adding phosphoramidite nucleosides to the 5′ terminus of said modified nucleoside to form a nucleic acid;
• c) adding a modified phosphoramidite nucleoside to the 5′ terminus of said nucleic acid;
• and
• d) adding an electron donor moiety to one modified nucleoside and an electron acceptor moiety to the other modified nucleoside.

A method for making a nucleic acid with electron transfer moieties attached at the 3′ terminus and an internal position, said method comprising:

• a) attaching a first modified nucleoside to a solid support;
• b) adding phosphoramidite nucleosides to the 5′ terminus of said modified nucleoside to form a nucleic acid;
• c) adding a second modified nucleoside to the 5′ terminus of said nucleic acid;
• d) adding additional phosphoramidite nucleosides to the 5′ terminus of said second modified nucleoside;
• and
• e) adding an electron donor moiety to one modified nucleoside and an electron acceptor moiety to the other modified nucleoside.

A method for making a nucleic acid with electron transfer moieties attached at the 5′ terminus and an internal position, said method comprising:

• a) attaching a nucleoside to a solid support;
• b) adding phosphoramidite nucleosides to the 5′ terminus of said modified nucleoside to form a nucleic acid;
• c) adding a first modified nucleoside to the 5′ terminus of said nucleic acid;
• d) adding additional phosphoramidite nucleosides to the 5′ terminus of said second modified nucleoside;
• e) adding a second modified nucleoside to the 5′ terminus of said nucleic acid;
• and
• f) adding an electron donor moiety to one modified nucleoside and an electron acceptor moiety to the other modified nucleoside.