Quality control method of DNA microarray

Abstract

A quality control method of a DNA microarray is provided which includes preparing a DNA spotting solution containing a first fluorescent dye having particular excitation and emission wavelengths, applying the DNA spotting solution to a substrate of a DNA chip to form DNA spots in which the first fluorescent dye is bound to the substrate, and detecting fluorescent signals from the DNA spots. In the DNA microarray quality control method, since a signal from a fluorescent dye covalently bound to the solid chip surface together with DNA probe is used for the quality control, staining and dye-removing processes, which were performed in conventional quality control methods, are unnecessary.

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