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US 2003/0170670 A1Published application
Novel glutaminase, its gene and a method of producing it
AssigneeAjinomoto Co., Inc.
InventorsKyoko Koibuchi, Hiroaki Nagasaki, Ari Yuasa, Jiro Kataoka, Katsuhiko Kitamoto
Life of this patent
Estimating the term…
Abstract
Glutaminase is purified from an Aspergillus oryzae, its partial amino acid sequence is determined, a partial sequence of glutaminase gene is obtained by PCR based on the obtained information, and DNA fragments containing glutaminase gene from Aspergillus oryzae genomic library and cDNA library, and Aspergillus nidulans genomic library by hybridization using the partial sequence as a probe.
Claims9
A protein defined in any of following (A) to (D):
- (A) a protein having an amino acid sequence represented by the amino acid numbers 1-670 of SEQ ID NO:
- 2 in Sequence Listing;
- (B) a protein having an amino acid sequence represented by the amino acid numbers 1-669 of SEQ ID NO:
- 22 in Sequence Listing;
- (C) a protein having an amino acid sequence represented by the amino acid numbers 1-670 of SEQ ID NO:
- 2 in Sequence Listing with substitution, deletion, insertion, addition or inversion of one or a plurality of amino acids, and having activity for catalyzing hydrolysis of glutamine to glutamic acid and ammonia;
- (D) a protein having an amino acid sequence represented by the amino acid numbers 1-669 of SEQ ID NO:
- 22 in Sequence Listing with substitution, deletion, insertion, addition or inversion of one or a plurality of amino acids, and having activity for catalyzing hydrolysis of glutamine to glutamic acid and ammonia.
5 more elements
A DNA which encodes a protein defined in any of the following (A) to (D):
- (A) a protein having an amino acid sequence represented by the amino acid numbers 1-670 of SEQ ID NO:
- 2 in Sequence Listing;
- (B) a protein having an amino acid sequence represented by the amino acid numbers 1-669 of SEQ ID NO:
- 22 in Sequence Listing;
- (C) a protein having an amino acid sequence represented by the amino acid numbers 1-670 of SEQ ID NO:
- 2 in Sequence Listing with substitution, deletion, insertion, addition or inversion of one or a plurality of amino acids, and having activity for catalyzing hydrolysis of glutamine to glutamic acid and ammonia;
- (D) a protein having an amino acid sequence represented by the amino acid numbers 1-669 of SEQ ID NO:
- 22 in Sequence Listing with substitution, deletion, insertion, addition or inversion of one or a plurality of amino acids, and having activity for catalyzing hydrolysis of glutamine to glutamic acid and ammonia.
5 more elements
Dependencies are inferred from the claim text.