Method of nucleic acid recovery

Abstract

For reducing side effects of blood transfusion, white (nucleated) blood cells are separated from donated human blood by filtering the blood through filter media selectively adsorbing white blood cells. Such filter media are processed for recovering nucleic acids contained in the white blood cells. The cells retained by the filter media are separated from the filter media e.g. by washing with an aqueous solution. The separated cells are then lysed and the nucleic acids are isolated from the components of the lysed cells. Preferably each portion of filter medium is used for filtering the blood of one individual donor and is processed separately whereby samples of nucleic acids of one individual each are obtained.

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