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OwnerToshihiro Mori, Yumiko Takeshita +1inventors
InventorsToshihiro Mori, Yumiko Takeshita, Yoshihiko Makino
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An object of the present invention is to provide: a method for isolating and purifying nucleic acids which employs a solid phase wherein the solid phase has excellent isolating capability, good washing efficiency, and easy workability, and can be mass produced with substantially identical isolating capability, the solid phase being used in a method for isolating and purifying nucleic acids by adsorbing nucleic acids in a sample onto a solid phase surface and desorbing the nucleic acids by washing and the like; and a unit for isolation and purification of nucleic acid which is suitable for carrying out said method. The present invention provides a method for isolating and purifying a nucleic acid, comprising the step of: adsorbing a nucleic acid onto a solid phase composed of an organic high polymer having a hydroxide group on a surface thereof, and desorbing the nucleic acid from the solid phase, and a unit for isolation and purification of nucleic acid comprising a container having at least two openings wherein the container contains a solid phase composed of organic high polymers having a hydroxyl group on a surface thereof.
A method for isolating and purifying a nucleic acid, comprising the step of:
2 The method for isolating and purifying a nucleic acid according to claim 1, wherein the organic high polymer having a hydroxide group on a surface thereof is surface-saponified cellulose acetate.
The method for isolating and purifying a nucleic acid according to claim 2, wherein the surface saponification rate of cellulose acetate is 5% or more.
The method for isolating and purifying a nucleic acid according to claim 2, wherein the surface saponification rate of cellulose acetate is 10% or more.
The method for isolating and purifying a nucleic acid according to claim 2, wherein the cellulose acetate is a porous membrane.
The method for isolating and purifying a nucleic acid according to claim 2, wherein the cellulose acetate is a non-porous membrane.
The method for isolating and purifying a nucleic acid according to claim 2, wherein the cellulose acetate is coated on beads.
A unit for isolation and purification of nucleic acid comprising a container which has at least two openings and contains a solid phase composed of an organic high polymer having a hydroxyl group on a surface thereof.
A unit for isolation and purification of nucleic acid comprising:
A method for incorporating a hydroxyl group into cellulose acetate, comprising the steps of coating beads with cellulose acetate, and saponifying the surface.
A bead having cellulose acetate membrane on a surface thereof, wherein the cellulose acetate membrane has a hydroxyl group incorporated thereto by surface-saponification.
Dependencies are inferred from the claim text.