Method for screening substance specifically inhibiting non-mevalonate pathway
OwnerHaruo Seto, Tomohisa Kuzuyama +1inventors
InventorsHaruo Seto, Tomohisa Kuzuyama, Nobuo Mitsui
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Abstract
The present invention provides a method for screening substances which specifically inhibit the non-mevalonate pathway by using an organism capable of using both the mevalonate pathway and the non-mevalonate pathway as biosynthetic pathways of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). According to the present invention, it is possible to provide a novel method for screening anti-bacterial agents, herbicides or anti-malarial agents which can specifically inhibit any of the enzyme reactions on the non-mevalonate pathway for the synthesis of isopentenyl diphosphate and dimethylallyl diphosphate (DMAPP).
Claims22
A method for screening substances which specifically inhibit the non-mevalonate pathway by using an organism capable of using both the mevalonate pathway and the non-mevalonate pathway as biosynthetic pathways of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).
A transformant which is obtained by introducing DNA encoding all enzymes necessary for using the mevalonate pathway as a biosynthetic pathway of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) in the presence of mevalonic acid, into a host having the non-mevalonate pathway.
Dependencies are inferred from the claim text.