Method for identifying a nucleic acid sequence
OwnerYi Liu, Pascal Bouffard +1inventors
InventorsYi Liu, Pascal Bouffard, Oswald Crasta
Life of this patent
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Abstract
The present invention provides methods by which biologically derived DNA sequences in a mixed sample or in an arrayed single sequence clone can be determined and classified without sequencing. The methods make use of information on the presence of carefully chosen target subsequences, typically of length from 4 to 8 base pairs, the length between target subsequences, and the measurement of the presence or absence of at least one additional target subsequence in a sample DNA sequence together with DNA sequence databases containing lists of sequences likely to be present in the sample to determine a sample sequence.
Claims13
A method of characterizing a polynucleotide sequence, the method comprising:
- (a) providing a linear nucleic acid sequence of known length with a defined 5′ terminus and a defined 3′ terminus, wherein said 5′ and 3′ termini are restriction endonuclease cleavage sites;
- (b) contacting said linear nucleic acid sequence with a first restriction endonuclease;
- and
- (c) determining whether said first restriction endonuclease cleaves said linear nucleic acid sequence, thereby characterizing said polynucleotide sequence.
A method of identifying a polynucleotide sequence, the method comprising:
- (a) providing information for a first linear nucleic acid sequence, wherein said information comprises:
- (i) the length of the first linear nucleic acid sequence
- (ii) a defined 5′ terminus and a defined 3′ terminus, wherein said 5′ and 3′ termini are restriction endonuclease cleavage sites;
- and
- (iii) cleavage status for at least one additional restriction endonuclease is known;
- (b) comparing said information of said first linear nucleic acid sequence to information for a second linear nucleic sequence wherein similarity of said information of the first linear nucleic acid sequence to said information of the second linear nucleic acid sequence indicates said first linear nucleic acid sequence is the second linear nucleic thereby identifying a polynucleotide sequence.
Dependencies are inferred from the claim text.