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AssigneeInstitut Pasteur
InventorsLuc Montagnier, Solange Chamaret, Denise Guetard, Marc Alizon, Francois Clavel, Mireille Guyader, Pierre Sonigo, Francoise Brun-Vezinet, Marianne Rey, Christine Rouzioux, Christine Katlama
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The invention relates to a new class of retroviruses, designated by HIV-2, of which samples have been deposited to the ECACC under numbers 87.01.1001 and 87.01.1002 and to the NCIB under numbers 12.398 and 12.399. It relates also to antigens capable to be obtained from this virus, particularly proteins p12, p16, p26 and gp140. These various antigens can be used for the diagnosis of the disease, especially by contacting these antigens with a serum of a patient submitted to the diagnosis. It relates to immunogenic compositions containing more particularly the glycoprotein gp140. Finally it concerns nucleotidic sequences, which can be used especially as hybridization probes, derived from the RNA of HIV-2.
HIV-2 retrovirus or variance of this virus, which retrovirus has infectious properties with respect to human T4 lymphocytes and the essential morphological and immunological properties of any of the retroviruses deposited at the CNCM under n• I-502, I-532, I-642 and I-643:
An antigen which provides a single bound in electrophoresis on a polyacrylamid gel which comprises, in common with one of the purified antigens of HIV-2 retrovirus, an epitope that is recognized by the serum of a carrier of antibody against HIV-2.
A purified antigen having the immunological characteristics of one of the following proteins or glycoproteins of HIV-2:
The kit of claim 21, whose means for detecting the immunological complexe formed comprises human anti-immunoglobulins or a protein A and a means for detecting the complexe formed between the anti-HIV-2 antibodies contained in the detected immunological conjuguate.
Immunogenic compositions containing an envelope glycoprotein of HIV-2 retrovirus, such as gp140 of said retrovirus, or part of said glycoprotein, in association with a pharmaceutically acceptable vehicle appropriate for the constitution of vaccines effective against HIV-2.
Nucleic acids, optionally labelled, derived of part at least of RNA of HIV-2 virus or of one of its variance.
A process for the production of HIV-2 retrovirus which comprises culturing human T4 lymphocytes or permanent cell lines derived from said T4 lymphocytes and carrying the T4 phenotype, which lymphocytes or cell lines had previously been infected with an isolate of HIV-2 virus and, particularly when the level of reverse transcriptase activity has reached a determined threshold, recovering and purifying the amounts of virus released in the culture medium of said lymphocytes or cell lines, particularly by differential centrifugation in a gradient of sucrose or metrizamide.
A process for the production of specific antigen of HIV-2 retrovirus which comprises lysing, particularly by means of detergent such as SDS (for instance 0.1% SDS in a RIPA buffer) and recovering the lysate containing said antigens;
Process for the production of one of the above defined proteins (p12, p16 or p26) or of a protein having the structure of gp140 or of determined parts of said proteins, which process comprises inserting the corresponding nucleic acid sequence in a vector capable of transforming an appropriate host,enabling the expression of an insert containing in said vector, transforming said host by said vector which comprises the said nucleotidic sequence, culturing the transformed cell lines host, recovering and purifying the expressed protein.
Dependencies are inferred from the claim text.