Probes and primers for the detection of polyphosphate accumulating organisms in wastewater
OwnerGregory Robert Crocetti, Gene William Tyson +2inventors
InventorsGregory Robert Crocetti, Gene William Tyson, Philip Hugerholtz, Linda Louise Blackall
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Abstract
The present invention relates to the identification of polyphosphate accumulating organisms that are capable of biologically removing phosphorus from wastewater. Specifically, the invention provides oligonucleotide probes or primers for detecting a polyphosphate accumulating organism in a sample. These oligonucleotides have a sequence of at least 12 nucleotides that is unique to 16S rDNA of polyphosphate accumulating organisms. The invention further provides methods for detecting polyphospbate accumulating organisms.
Claims12
An oligonucleotide probe or primer for detecting a polyphosphate accumulating organism in a sample, said oligonucleotide having a sequence of 12 to 50 nucleotide selected from any one of SEQ ID NO. 5 to SEQ ID NO. 9 or the reverse complement of any one of SEQ ID NO. 5 to SEQ ID NO. 9;
- and wherein said oligonucleotide has the binding characteristics of an oligonucleotide of any one of the following sequences:
- 8 5′-CCGTCATCTACWCAGGGTATTAAC-3′ (SEQ ID NO. 11) 5′-CCCTCTGCCAAACTCCAG-3′ (SEQ ID NO. 12) 5′-GTTAGGTACGGCACTAAAAGG-3′. (SEQ ID NO. 13)
An oligonucleotide probe or primer for detecting organism in a sample related to polyphosphate accumulating organisms, said oligonucleotide having a sequence of 12 to 50 nucleotides selected from any one of the sequences of FIG. 3 (SEQ ID NO. 1 to SEQ ID NO. 10) or the reverse complement of any one of the sequences of FIG. 3;
- aid wherein said oligonucleotide has the binding characteristics of an oligonucleotide of the following sequence:
- 5′-AGGATTCCTGACATGTCAAAGGG-3′ (SEQ ID NO. 14).
Dependencies are inferred from the claim text.