Method of detecting target base sequence
AssigneeKatayanagi Institute
InventorsIsao Karube, Teru Kato
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Abstract
A method for detecting nucleic acids wherein the binding activity of a nucleic acid aptamer, which is formed by the hybridization of a target nucleotide sequence and a probe, is detected using the binding with a ligand as an index has been provided. Utilizing a structure such as the three-way junction, whose binding activity significantly changes depending on a mismatch of a single nucleotide, as the nucleic acid aptamer SNP can be detected.
Claims8
A method for detecting a target nucleotide sequence, which comprises the steps of:
- (a) hybridizing a probe to the target nucleotide sequence to form a nucleic acid aptamer having the binding affinity for a ligand;
- and
- (b) detecting the presence of the target nucleotide sequence using the affinity of the aptamer as an index.
A probe that acquires the binding affinity for a ligand by the hybridization with the target nucleotide sequence.
A nucleic acid aptamer, which has three or more stems and binds with the ligand at the position where the stems intersect.
Dependencies are inferred from the claim text.