Method for detecting and evaluating a potentially aberrantly methylated dna region on the x chromosome, or the clonality

A method for detecting and evaluating a potentially aberrantly methylated DNS region on the X chromosome, or the clonality, characterized in that

• a) either the extent of methylation of the potentially aberrantly methylated DNA region and/or the extent of methylation in a definitely physiologically methylated DNA region of the X chromosome is determined in a sample and the presence and optionally the variant and the extent of the potential aberration are subsequently diagnosed from the comparison of the methylation determinations, or
• b) the extent of methylation of the potentially aberrantly methylated DNA region and/or the extent of methylation, as well as optionally the sequence variant, of a polymorphous DNA region of the X chromosome, which may either be physiologically or aberrantly methylated, is determined in a sample, and the presence and optionally the variant and the extent of the potential aberration, or the clonality, are subsequently diagnosed from the comparison of the methylation determinations and optionally the sequence variant, or
• c) the extent of methylation of the potentially aberrantly methylated DNA region, the extent of methylation in a definitely physiologically methylated DNA region of the X chromosome, and the extent of methylation as well as the sequence variant of a polymorphous DNA region of the X chromosome, which may either be physiologically or aberrantly methylated, are determined in a sample, and the presence and optionally the variant and the extent of the potential aberration are subsequently diagnosed from the comparison of the methylation determinations and the sequence variant.