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AssigneeGenset S.A.
InventorLydie Bougueleret
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The present invention is directed to a polynucleotide comprising open reading frames defining a coding region encoding a retinoblastoma binding protein (RBP-7) as well as regulatory regions located both at the 5′en d and the 3′ end of said coding region. The present invention also pertains to a polynucleotide carrying the natural regulation signals of the RBP-7 gene which is useful in order to express a heterologous nucleic acid in host cells or host organisms as well as functionally active regulatory polynucleotides derived from said regulatory region. The invention also concerns polypeptides encoded by the coding region of the RBP-7 gene. The invention also deals with antibodies directed specifically against such polypeptides that are useful as diagnostic reagents. The invention also comprises genetic markers, namely biallelic markers, that are means that may be useful for the diagnosis of diseases related to an alteration in the regulation or in the coding regions of the RBP-7 gene and for the prognosis/diagnosis of an eventual treatment with therapeutic agents, especially agents acting on pathologies involving abnormal cell proliferation and/or abnormal cell differentiation
A purified or isolated polynucleotide comprising a contiguous span of at least 12 nucleotides of SEQ ID No. 1 or the complements thereof, wherein said contiguous span comprises at least 1 of the following nucleotide positions of SEQ ID No. 1:
2 A purified or isolated polynucleotide comprising a contiguous span of at least 12 nucleotides of SEQ ID No. 4 or the complements thereof, wherein said contiguous span comprises at least I of the following nucleotide positions of SEQ D No. 4:
A purified or isolated nucleic acid comprising a nucleotide sequence selected >from the group of SEQ ID Nos. 5-28, a sequence complementary thereto or a fragment or a variant thereof.
A purified or isolated nucleic acid comprising a combination of at least two polynucleotides selected from the group consisting of SEQ ID Nos. 5-28, wherein the polynucleotides are arranged within the nucleic acid, from the 5′ end to the 3′end of said nucleic acid, in the same order than in SEQ ID No 1
An oligonucleotide of at least 8 nucleotides in length that hybridizes under stringent hybridization conditions with a nucleic acid selected from the group consisting of the nucleotide sequences 1-481, 666-1465, 1521-67592, 67704-71118, 71185-72598, 72690-75543, 75624-81841, 81934-83019, 83406-87901, 88041-93856, 93937-97158, 97236-98962, 99086-103188, 103745-104303, 104654-105084, 105180-106682, 106781-107798, 107897-108392, 108552-114335, 114418-114491, 114594-132246, 132332-134150, 134350-145565, 145842-146332, 146775-150446, 150542-152959, 153176-155590, 155738-159701, 160466-161028, and 161453-162450 of SEQ ID No 1 or a sequence complementary thereto
An oligonucleotide of at least 8 nucleotides in length that hybridizes under stringent hybridization conditions with a nucleic acid selected from the group consisting of the nucleotide sequences 1-208, 1307-1350, 1703-1865, 2107-2180, 2843-3333, 3871-3882, 4222-4276, and 5017-5579 of SEQ ID No. 4 or a sequence complementary thereto
An isolated, purified, or recombinant polynucleotide consisting essentially of a contiguous span of 8 to 50 nucleotides of any one of SEQ ID Nos. 1 and 4 and the complement thereof, wherein said span includes a biallelic marker of RBP-7.
An isolated, purified, or recombinant polynucleotide consisting essentially of a contiguous span of 8 to 50 nucleotides of any one of SEQ ID Nos 1 and 4 and the complement thereof, wherein the 3′ end of said contiguous span is located at the 3′ end of said polynucleotide, and wherein the 3′ end of said polynucleotide is located within 20 nucleotides upstream of a biallelic marker of RBP-7 in said sequence
16 An isolated, purified, or recombinant polynucleotide consisting essentially of a sequence selected from the sequences of SEQ ID Nos. 72-101
An isolated, purified, or recombinant polynucleotide which encodes a polypeptide comprising a contiguous span of at least 6 amino acids of SEQ ID No. 29.
18 An isolated, purified, or recombinant polynucleotide for use in hybridization assays, sequencing assays, and enzyme-based mismatch detection assays for determining the identity of the nucleotide at a biallelic marker of RBP-7.
A solid support having a polynucleotide attached thereto, wherein said polynucleotide is selected from the group consisting of
21 An array according to claim 20, wherein said array is addressable.
A polynucleotide having a label thereon, wherein said polynucleotide is selected from the group consisting of.
A recombinant vector comprising a polynucleotide selected from the group consisting of:
A mammalian host cell comprising a RBP-7 gene disrupted by homologous recombination with a knock out vector, comprising a polynucleotide selected from the group consisting of:
A non-human host mammal comprising a RBP-7 gene disrupted by homologous recombination with a knock out vector, comprising a polynucleotide selected from the group consisting of:
29 An isolated or purified antibody composition capable of selectively binding to an epitope-containing fragment of a polypeptide according to claim 28
Dependencies are inferred from the claim text.