Methods for screening for transdominant effector peptides and RNA molecules
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsGarry P. Nolan, S. Michael Rothenberg
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Abstract
Methods and compositions for screening for transdominant effector peptides and RNA molecules selected inside living cells from randomized pools are provided.
Claims27
A method for screening for a transdominant bioactive agent capable of altering the phenotype of a cell, said method comprising the steps:
- a) introducing a molecular library of randomized candidate nucleic acids into a plurality of cells, wherein each of said nucleic acids comprises a different nucleotide sequence;
- b) screening said plurality of cells for a cell exhibiting an altered phenotype, wherein said altered phenotype is due to the presence of a transdominant bioactive agent.
A method for screening for a transdominant bioactive agent capable of altering the phenotype of a cell, said method comprising the steps:
- a) introducing a molecular library of randomized candidate nucleic acids into a first plurality of cells, wherein each of said nucleic acids comprises a different nucleotide sequence;
- b) contacting said first plurality of cells with a second plurality of cells;
- and
- c) screening said second plurality of cells for a cell exhibiting an altered phenotype.
A molecular library of retroviruses comprising at least 104 different randomized nucleic acids.
A cellular library of mammalian cells containing a molecular library of retroviral constructs, said molecular library comprising at least 104 different randomized nucleic acids.
Dependencies are inferred from the claim text.