Acyl fluoride activation of carboxysilyl-coated glass substrates

A method of covalently immobilizing nucleophile-containing species on glass, comprising:

• (a) providing a nucleophile-containing species;
• (b) providing a glass substrate having a surface with at least one pendant carboxysilyl linker comprising a carboxyl group;
• (c) activating the surface of the glass by converting the carboxyl group into an acyl fluoride group;
• (d) neutralizing a hydrogen fluoride side product of the activating step (c) to prevent etching of the glass surface;
• and
• (e) contacting the nucleophile-containing species with the activated glass surface under a condition that allows covalent binding of the nucleophile-containing species with the acyl fluoride group.

A bioarray prepared by a method comprising the steps of:

• (a) providing a plurality of biomolecules;
• (b) providing a glass substrate having a surface with at least one pendant carboxysilyl linker comprising a carboxyl group;
• (c) activating the surface of the glass by converting the carboxyl group into an acyl fluoride group;
• (d) neutralizing a hydrogen fluoride side product of the activating step (c) to prevent etching of the glass surface;
• and
• (e) contacting the biomolecules with the activated glass surface at discrete locations under a condition that allows a covalent binding of the biomolecules with acyl fluoride groups to form a bioarray.