Slotted electrophoresis gel composition and methods of use thereof
AssigneeExact Sciences Corporation
InventorsPhilip W. Hammond, Christopher P. Adams, Ezra S. Abrams, T. Christian Boles
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Abstract
A slotted electrophoresis gel composition and methods of use are disclosed in the present invention. The invention also describes an apparatus that is used to produce a slotted electrophoresis gel composition.
Claims20
A slotted electrophoresis gel composition comprising a primary gel comprising a first matrix with a sample loading zone and at least one preformed slot comprising an opening for a secondary gel comprising a second matrix, wherein said slot can have any geometric configuration within the primary gel.
An apparatus for producing a slotted electrophoresis gel composition comprising the following:
- (a) at least one slot-forming comb, wherein said slot-forming comb is comprised of at least one slot-forming element;
- (b) two plates that serve to constrain the electrophoresis medium when in the liquid phase;
- (c) at least one spacer that is positioned between the two plates of (b), and
- (d) at least two clamps that secure the two plates of (c) together.
A diagnostic kit for determining the presence, or absence, of a target molecule in a biological sample comprising:
- (i) an electrophoresis gel composition comprising a primary gel comprising a first matrix with a sample loading zone and at least one preformed slot opening for a secondary gel, wherein said slot can have any geometric configuration within the primary gel, and
- (ii) optionally one, or more, reagent vials comprising one, or more, second matrices comprising reagents specific for the detection of one, or more, target molecules in a biological sample, wherein these reagents are immobilized to a medium.
Diagnostic kit reagents comprising matrix material comprising immobilized probes specific for target molecules.
Dependencies are inferred from the claim text.