Nucleic acid capable of promoting gene expression
OwnerToshio Harainventor
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Abstract
Provided is a nucleic acid capable of inducing or promoting the expression of a gene. The present invention relates to a nucleic acid capable of inducing or promoting the expression of a gene. The present invention provides, among other things, a secretory signal sequence of a chicken lysozyme or a secretory signal sequence of an SF162 type HIVgp120. When the SF2 type HIVgp120 has an intrinsic signal sequence, it is difficult to artificially express the gene. The expression thereof, however, can be induced by replacing the intrinsic signal sequence with the two secretory signal sequences.
Claims23
A nucleic acid inserted into the upstream of a gene linked expressibly to a promoter, wherein said nucleic acid is capable of implementing or promoting the expression of said gene from said promoter and wherein said nucleic acid is a secretory signal sequence of a chicken lysozyme or a secretory signal sequence of an SF162 type human immunodeficiency virus (HIV) gp120.
An expression cassette for implementing or promoting the expression of a gene, comprising:
- a promoter connected expressibly to said gene;
- a secretory signal sequence disposed upstream of said gene and expressed integrally with said gene from said promoter;
- and a 3′UTR disposed downstream of said gene, wherein said secretory signal sequence is a secretory signal sequence of a chicken lysozyme or a secretory signal sequence of an SF162 type human immunodeficiency virus (HIV) gp120.
An SF2 type HIVgp120 expression cassette, comprising:
- said SF2 type HIVgp120;
- a secretory signal sequence linked to the upstream of said SF2 type HIVgp120;
- a promoter connected to the upstream of said SF2 type HIVgp120 with said secretory signal sequence therebetween so as to express the SF2 type HIVgp120 to which said secretory signal sequence is linked;
- and a 3′UTR connected to the downstream of said SF2 type HIVgp120, wherein said secretory signal sequence is a secretory signal sequence of a chicken lysozyme or a secretory signal sequence of an SF162 type human immunodeficiency virus (HIV) gp120.
A method for determining whether a secretory signal sequence, which is a test sample, is capable of implementing or elevating the expression of a gene, said method comprising:
- preparing an expression vector by connecting a monitor gene between a promoter and a 3′UTR on the vector, to which said secretory signal sequence is linked upstream so as to enable the expression from said promoter;
- introducing said expression vector into the cell;
- and measuring the transcriptional product of said monitor gene in said measurement step.
A system for determining whether a secretory signal sequence, which is a test sample, is capable of elevating the expression of a gene, said system comprising:
- (i) an expression vector which is connected to said gene and which has a promoter and a 3′UTR for connecting a monitor gene to which said secretory signal sequence is linked upstream so as to be expressible from said promoter and (ii) a cell for expressing said monitor gene by introducing said expression vector, wherein said system determines whether the nucleic acid, which is said test sample, is capable of elevating the expression of the monitor gene based on the representation of said monitor gene in said cell.
Dependencies are inferred from the claim text.