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US 2003/0170606 A1Published application
High-throughput screening assays for modulators of mitochondrial membrane potential
OwnerShu-Gui Huang, Jin-Long Cheninventors
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Abstract
The present invention provides methods for identifying modulators of uncoupling activity in mitochondria and for modulators of uncoupling proteins. In particular, this invention provides homogeneous assays for screening one or more test agents for the ability to modulate uncoupling activity in vivo.
Claims45
A method of screening a test agent for an ability to modulate the activity of an uncoupling protein, said method comprising:
- (i) expressing said uncoupling protein in a cell;
- (ii) introducing a fluorescent probe into said cell, wherein the fluorescence of said cell in the presence of said fluorescent probe is a function of the membrane potential in the mitochondria in said cell;
- (iii) contacting said cell with said test agent;
- and
- (iv) detecting the fluorescence in said cell;
- wherein an alteration in the fluorescence in said cell in the presence of said test agent compared to the fluorescence in the absence of said test agent indicates an ability of said test agent to modulate said activity of said uncoupling protein;
- and
- wherein said screening is performed in a homogeneous format.
5 more elements
A method of screening a test agent for an ability to modulate uncoupling activity in mitochondria, comprising:
- (i) introducing a fluorescent probe into a cell, wherein the fluorescence of said fluorescent probe in said cell is a function of the membrane potential in said mitochondria;
- (ii) contacting said cell with said test agent;
- and
- (iii) detecting the fluorescence in said cell;
- wherein an alteration in the fluorescence in the cell in the presence of the test agent compared to the fluorescence in the cell in the absence of the test agent indicates an ability of the test agent to modulate uncoupling activity;
- and
- wherein said screening is performed in a homogeneous format.
4 more elements
Dependencies are inferred from the claim text.