Substrate for protein microarray containing functionalized polymer

A gelatin-based substrate for fabricating protein arrays, the substrate comprising:

• gelatin having at least one surface;
• a polymer scaffold affixed to the gelatin surface;
• and
• a trifunctional compound A-L-B;
• wherein A is a functional group capable of interacting with the polymer scaffold;
• L is a linking group capable of interacting with A and with B;
• and B is a specific functionality that provides one or more reactive units capable of interacting with a protein capture agent.

A method of making a gelatin-based substrate for fabricating protein arrays comprising the steps of:

• providing a support;
• coating on the support a composition containing gelatin;
• bonding a polymer scaffold to a surface of the gelatin;
• wherein the polymer in the scaffold is rich in reactive units capable of immobilizing proteins.

A method of making a gelatin-based substrate for fabricating protein arrays comprising the steps of:

• providing a support;
• coating on the support a composition containing gelatin;
• affixing a polymer scaffold to a surface of the gelatin;
• and
• bonding a trifunctional compound A-L-B to the polymer scaffold;
• wherein A is a functional group capable of bonding to the polymer scaffold;
• L is a linking group capable of connecting A with B;
• and B is a reactive unit that provides one or more reactive units capable of interacting with a protein or protein capture agent.