Thermotolerant phytase for animal feed
AssigneeSyngenta Participations AG
InventorsMichael B. Lanahan, Scott Betts
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Abstract
The invention provides a synthetic phytase polynucleotide which is optimized for expression in plants and which encodes at thermotolerant phytase, as well as isolated thermotolerant phytase enzyme. Also provided are feed or food products comprising a thermotolerant phytase, and transgenic plants which express the thermotolerant phytase. Further provided are methods for making and using thermotolerant phytases, e.g., a method of using a thermotolerant phytase in feed and food processing.
Claims56
A method to prepare a thermotolerant phytase comprising:
- expressing in a plant cell an expression cassette comprising a promoter operably linked to a nucleic acid molecule encoding a thermotolerant phytase which retains at least 40% activity after 30 minutes at 60° C. and has a specific activity of greater than 200 U/mg at pH 4.5 and 37° C.
A method of minimizing dietary requirements of phosphorus in an animal comprising:
- feeding to an animal a feed comprising the thermotolerant phytase of
Dependencies are inferred from the claim text.