Methods for preparing an estrogenic preparation and isolated estrogenic compounds from a plant and uses thereof
AssigneeNational University of Singapore
InventorsEu Leong Yong, Sook Peng Yap
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Abstract
Methods for preparing an estrogenic preparation and isolating estrogenic compounds from a plant, such as an Epimedium plant, are provided. Also provided are estrogenic compounds from Epimedium plant that have been isolated and characterized, and methods for their use in modulating estrogen receptors and in treating conditions mediated by estrogen receptors, such as menopause and estrogen-dependent cancers. Also provided are preparations from Epimedium that are enriched for estrogenic compounds, and methods for their use in modulating estrogen receptors and preventing and treating conditions that are mediated by estrogen receptors.
Claims51
A compound having the formula
- 10
A method for modulating an estrogen receptor comprising contacting said estrogen receptor with a compound having the formula
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A compound having the formula
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A method for preparing a preparation having estrogenic activity from a plant, said method comprising:
- (a) extracting the plant or a part thereof with a solvent to obtain an extract;
- (b) fractionating said extract to obtain a plurality of fractions;
- (c) assaying estrogen receptor agonist activity in the fractions from (b);
- and
- (d) collecting a fraction from (c) that is enriched for estrogen receptor agonist activity by about 2-fold or more relative to said extract.
A preparation having estrogenic activity obtained from an Epimedium plant, wherein said preparation comprises between about 0.002% to 99.9% by weight of a compound having the formula
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The preparation according to claim 32, wherein said preparation activates beta estrogen receptors (ER-beta) more strongly than alpha estrogen receptors (ER-alpha) by about 2-fold to 9-fold.
A method for preventing or treating a condition mediated by an estrogen receptor in a subject comprising administering to a subject in need thereof a therapeutically effective amount of:
- (i) a compound of the formula
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- (ii) a compound of the formula
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- (iii) a mixture of said compound in (i) and said compound in (ii);
- or
- (iv) a preparation having estrogenic activity obtained from an Epimedium plant, wherein said preparation comprises between about 0.002% to 99.9% of a compound of the formula
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Dependencies are inferred from the claim text.