Delivery of tissue engineering media

Abstract

The delivery of tissue engineering media using an airbrush. William S. Veazey, D M D Nov. 7, 2002 1 Average Delivery Values for Cell Delivery Study using Tissue Engineering Media Delivery System Fine Nozzle Medium Nozzle Large Nozzle 312 microns 494 microns 746 microns 6 PSI 86% 87% 94% 8 PSI 76% 82% 93% 10 PSI 65% 86% 89% 14 PSI 60% 66% 69% 18 PSI 37% 62% 56% Procedure: Bovine dermal fibroblasts were delivered into a 6-well tissue culture plate using a Badger 100 G airbrush having fine, medium and large nozzles at air pressures 6, 8, 10, 14 and 18 psi. Cells were delivered in a concentration of 200,000 cells per 1 mL of media; 1 mL of this cell suspension was delivered for each repetition. The cells were washed in Hanks Balanced Salt Solution (HBSS) or Phosphate Buffered Saline (PBS). Trypsin (0.25%) was used to detach the cells from the cell culture plate before delivery. Cells were re-suspended in a 50%-50% Dulbecco's Modified Eagle's Medium (DMEM) and F-12 media (one actually purchases the media in this concentration, called DMEM/F12) with 10% Fetal Bovine Serum (FBS), 2 mM L-Glutamine and 0.1 mM 2-Me. It should be stated that cells could be delivered in a variety of media, and one skilled in the art recognizes that one could use HBSS or PBS alone, or could use DMEM/F12 alone, or any other type of vital media to deliver cells. Cell vitality was measured using a trypan blue exclusion assay. Thickness of deposition was not measured. The 1 mL of airbrush delivered media spread in the tissue culture well as media would normally do.

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