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AssigneeThe Secretary of State for Defence
InventorsEthel Diane Williamson, Julie Miller, Nicola Jane Walker, Leslie William James Baillie, Paula Thomson Holden, Helen Claire Flick-Smith, Helen Lisa Bullifent, Richard William Titball, Andrew William Topping
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An immunogenic reagent which produces an immune response which is protective against Bacillus anthracis, said reagent comprising one or more polypeptides which together represent up to three domains of the full length Protective Antigen (PA) of B. anthracis or variants of these, and at least one of said domains comprises domain 1 or domain 4 of PA or a variant thereof. The polypeptides of the immunogenic reagent as well as full length PA are produced by expression from E. coli. High yields of polypeptide are obtained using this method. Cells, vectors and nucleic acids used in the method are also described and claimed.
An immunogenic reagent which produces an immune response which is protective against Bacillus anthracis, said reagent comprising one or more polypeptides which together represent up to three domains of the full length Protective Antigen (PA) of B. anthracis or variants of these, and at least one of said domains comprises domain 1 or domain 4 of PA or a variant thereof.
An immunogenic reagent according to any one of the preceding claims wherein a polypeptide is fused to a further polypeptide.
A nucleic acid which encodes a polypeptide of an immunogenic reagent according to any one of the preceding claims.
A method for producing an immunogenic polypeptide which produces an immune response which is protective against B. anthracis, said method comprising transforming an E. coli host with a nucleic acid which encodes either (a) the protective antigen (PA) of Bacillus anthracis or a variant thereof which can produce a protective immune response, or (b) a protective domain of the protective antigen (PA) of Bacillus anthracis or a variant thereof which can produce a protective immune response, culturing the transformed host and recovering the polypeptide therefrom, provided that where the polypeptide is the protective antigen (PA) of Bacillus anthracis a variant thereof which can produce a protective immune response, the percentage of guanidine and cytosine residues within the said nucleic acid is in excess of 35%.
A recombinant Escherichia coli cell which has been transformed with a nucleic acid which encodes the protective antigen (PA) of Bacillus anthracis or a variant thereof which can produce a protective immune response, and wherein the percentage of guanidine and cytosine residues within the nucleic acid is in excess of 35%.
A recombinant Escherichia coli cell which has been transformed with a nucleic acid which encodes a protective domain of the protective antigen (PA) of Bacillus anthracis or a variant thereof which can produce a protective immune response.
An E. coli transformation vector comprising a nucleic acid which encodes the protective antigen (PA) of Bacillus anthracis or a variant thereof which can produce a protective immune response, and wherein the percentage of guanidine and cytosine residues within the nucleic acid is in excess of 35%.
An E. coli transformation vector comprising a nucleic acid which encodes a protective domain of the protective antigen (PA) of Bacillus anthracis or a variant thereof which can produce a protective immune response.
A nucleic acid of SEQ ID NO 1 or a modified form thereof which encodes PA or a variant thereof which produces a protective immune response and which has at least 35% GC content.
Dependencies are inferred from the claim text.