Immunogenic detoxified mutant E. coli LT-A toxin
AssigneeChiron Corporation
InventorsMariagrazia Pizza, Marzia Monica Giuliani, Rino Rappuoli
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Abstract
An immunogenic detoxified protein is provided which comprises the amino acid sequence of subunit A of an E. coli heat labile toxin (LT-A) or a fragment thereof in which at least amino acid Ala-72 of the A subunits mutated, preferably by substitution with Arg. The toxoid is useful as vaccine against an enterotoxigenic strain of E. coli and is produced by recombinant DNA means by site-directed mutagenesis. It is also an effective adjuvant.
Claims15
An immunogenic detoxified protein comprising the amino acid sequence of subunit A of an E. coli heat labile toxin (LT-A), or a fragment thereof, wherein at least amino acid Ala-72 in said sequence or fragment is mutated.
Dependencies are inferred from the claim text.