Chlamydia antigens and corresponding dna fragments and uses thereof
AssigneeMintz, Levin, Cohen, Ferris, Glovsky, Popeo
InventorsANDREW D. MURDIN, RAYMOND P. OOMEN, PAMELA L. DUNN
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Abstract
In summary of this disclosure, the present invention provides a method of nucleic acid, including DNA, immunization of a host, including humans, against disease caused by infection by a strain of Chlamydia, specifically C. pneumoniae, employing a vector, containing a nucleotide sequence encoding an 98 kDa putative outer membrane protein of a strain of Chlamydia pneumoniae and a promoter to effect expression of the 98 kDa putative outer membrane protein gene in the host. Modifications are possible within the scope of this invention.
Claims30
The polypeptide of claim 7, wherein the fusion polypeptide is a signal peptide.
The polypeptide of claim 7, wherein the fusion polypeptide comprises a heterologous polypeptide having adjuvant activity.
An expression cassette, comprising the polynucleotide of claim 1 operably linked to a promoter.
A pharmaceutical composition, comprising an immunologically effective amount of the polypeptide of claim 5 and pharmaceutically acceptable diluent.
A polynucleotide probe reagent capable of detecting the presence of Chlamydia in biological material, comprising a polynucleotide that hybridizes to the polynucleotide of claim 1 under stringent conditions.
A method for detecting the presence of Chlamydia in a sample comprising the steps of:
- (a) contacting said sample with a detecting reagent that binds to the polypeptide having SEQ ID NO:
- 2 to form a complex;
- and
- (b) detecting said formed complex.
An affinity chromatography method for substantially purifying a polypeptide having SEQ ID NO:
- 2, comprising the steps of:
- (a) contacting a sample containing said polypeptide with a detecting reagent that binds to said polypeptide to form a complex;
- (c) isolating said formed complex;
- (c) dissociating said formed complex;
- and
- (d) isolating the dissociated polypeptide.
An antibody that immunospecifically binds a polypeptide of claim 5, or a fragment or derivative of said antibody containing the binding domain thereof.
Dependencies are inferred from the claim text.