Vascular endothelial growth factor-B and DNA coding therefor
AssigneeHelsinki University Licensing, Ltd.
InventorsUlf Eriksson, Birgitta Olofsson, Kari Alitalo, Katri Pajusola
Life of this patent
Estimating the term…
Abstract
VEGF-B polypeptides from the PDGF family of growth factors having the property of promoting mitosis and proliferation of vascular endothelial cells, DNA sequences encoding these polypeptides, pharmaceutical compositions containing them and antibodies which react with them. The VEGF-B polypeptides are useful in stimulating angiogenesis as well as in diagnostic applications.
Claims57
An isolated DNA which codes for a protein which comprises a characteristic amino acid sequence
- Pro-Xaa-Cys-Val-Xaa-Xaa-Xaa-Arg-Cys-Xaa-Gly-Cys-Cys (SEQ ID NO:16) and has the property of promoting proliferation of endothelial cells or mesodermal cells, said DNA being selected from the group consisting of the DNA of FIGS. 1 and 2 (SEQ ID NO:1), the DNA of FIG. 3 (SEQ ID NO:4), the DNA of FIG. 5 (SEQ ID NO:6), the DNA of FIG. 7 (SEQ ID NO:8), the DNA of FIG. 10 (SEQ ID NO:10), the DNA of FIG. 12 (SEQ ID NO:12), the DNA of FIG. 14 (SEQ ID NO:14), and DNA which hybridizes under stringent conditions with at least one of the foregoing DNA sequences.
A protein which exhibits a characteristic sequence
- Pro-Xaa-Cys-Val-Xaa-Xaa-Xaa-Arg-Cys-Xaa-Gly-Cys-Cys (SEQ ID NO:16) and has the property of promoting proliferation of endothelial cells or mesodermal cells, said protein comprising a sequence of amino acids substantially corresponding to an amino acid sequence selected from the group consisting of the amino acid sequence of FIG. 1 (SEQ ID NO:2), the amino acid sequence of FIG. 2 (SEQ ID NO:3), the amino acid sequence of FIG. 4 (SEQ ID NO:5), the amino acid sequence of FIG. 6 (SEQ ID NO:7), the amino acid sequence of FIG. 8 (SEQ ID NO:9), the amino acid sequence of FIG. 11 (SEQ ID NO:11), the amino acid sequence of FIG. 13 (SEQ ID NO:13), and the amino acid sequence of FIG. 15 (SEQ ID NO:15).
A protein produced by expression of a DNA selected from the group consisting of the DNA of FIGS. 1 and 2 (SEQ ID NO:1), the DNA of FIG. 3 (SEQ ID NO:4), the DNA of FIG. 5 (SEQ ID NO:6), the DNA of FIG. 7 (SEQ ID NO:8), the DNA of FIG. 10 (SEQ ID NO:10), the DNA of FIG. 12 (SEQ ID NO:12), the DNA of FIG. 14 (SEQ ID NO:14), and DNA which hybridizes under stringent conditions with at least one of the foregoing DNA sequences.
An isolated DNA comprising a nucleotide sequence corresponding to the sequence of FIG. 25 (SEQ ID NO:17) or a DNA which hybridizes under stringent conditions to said nucleotide sequence.
Dependencies are inferred from the claim text.