Antibody compositions specific for p33QIK and p63Krs1 polypeptides and uses thereof
OwnerHwa-Chain Robert Wanginventor
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Abstract
Disclosed are compositions and methods for the detection and quantitation of mammalian p33QIK and p63Krsl polypeptides in a sample. Also disclosed are methods, kits, and reagents for the diagnosis of diseases manifesting in or resulting from altered p33QIK and/or p63Krsl polypeptide levels in a patient.
Claims60
An isolated peptide of from 9 to about 70 amino acids in length, said peptide comprising at least a first contiguous amino acid sequence according to any one of SEQ ID NO:3 through SEQ ID NO:76.
An isolated polypeptide consisting essentially of the amino acid sequence from position 1 to position 322 of SEQ ID NO:2.
A monoclonal antibody that specifically binds to a native, or biologically active mammalian p33QIK or p63Krsl peptide or polypeptide, but does not bind to a mammalian p63Krsl peptide or polypeptide denatured under conditions of SDS-PAGE.
A composition comprising:
- (a) an isolated peptide of from 9 to about 70 amino acids in length, said peptide comprising at least a first contiguous amino acid sequence according to any one of SEQ ID NO:3 through SEQ ID NO:76;
- (b) an isolated polypeptide consisting essentially of the amino acid sequence from position 1 to position 322 of SEQ ID NO:2;
- (c) a native, or biologically-active mammalian p33QIK or p63Krsl peptide or polypeptide;
- or
- (d) a purified antibody, polyclonal antiserum, or antigen-binding fragment thereof, that is immunospecific for the peptide of (a), the polypeptide of (b), or the peptide of polypeptide of (c), or a hybridoma cell capable of producing said antibody.
A kit comprising:
- (a) (i) an isolated peptide of from 9 to about 70 amino acids in length, said peptide comprising at least a first contiguous amino acid sequence according to any one of SEQ ID NO:3 through SEQ ID NO:76;
- (ii) an isolated polypeptide consisting essentially of the amino acid sequence from position 1 to position 322 of SEQ ID NO:2;
- (iii) a native, or biologically-active mammalian p33QIK or p63Krsl peptide or polypeptide;
- or
- (iv) a purified antibody, polyclonal antiserum, or antigen-binding fragment thereof, that is immunospecific for the peptide of (i), the polypeptide of (ii), or the peptide of polypeptide of (iii), or a hybridoma cell capable of producing said antibody;
- and
- (b) instructions for using said kit.
Dependencies are inferred from the claim text.