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OwnerIchiro Masaki, Yoshikazu Yonemitsu +3inventors
InventorsIchiro Masaki, Yoshikazu Yonemitsu, Katsuo Sueshi, Mamoru Hasegawa, Hiroaki Kinoh
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Use of a recombinant Paramyxovirus vector has enabled transferring nucleic acid into the blood vessel at high efficiency by short exposure. The present invention provides a Paramyxovirus vector used for transferring nucleic acid into the vascular cells and a method for transferring nucleic acid using such vector. Pretreatment of blood vessels with protease significantly improved the efficiency of transfection into the medial layer. The expression of transfected genes was stable in the vascular cells for a long period. The method of the present invention enables efficiently transferring genes into tissues such as the blood vessel lumen, media, and adventitia in short time in such applications as gene therapy.
A method for transferring a nucleic acid into a vascular cell, said method comprising a step of contacting a recombinant Paramyxovirus vector or a cell comprising said vector with said vascular cell.
A method for transferring a nucleic acid into a vascular cell, said method comprising performing the following steps (a) and (b) sequentially or simultaneously:
A recombinant Paramyxovirus vector that transfers a nucleic acid into a vascular cell.
Dependencies are inferred from the claim text.