Method of purifying a hydrophobin present in a hydrophobin-containing solution
AssigneeApplied NanoSystems B.V.
InventorsMarcel Leo de Vocht, Herman Abel B. Wosten, Joseph Gerard H. Wessels
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Abstract
The invention relates to a method of purifying a hydrophobin present in a hydrophobin-containing solution. According to the invention the solution is contacted with a surface for the adsorption to that surface, and separated from a solution depleted in hydrophobin. Subsequently the surface is contacted with a solution containing a surfactant at a temperature lower than 90° C. Desorbed hydrophobin is separated from said surface.
Claims6
Method of purifying a hydrophobin present in a hydrophobin-containing solution, characterized in that the hydrophobin-containing solution is contacted with a surface for adsorption to that surface, separation of the surface carrying adsorbed hydrophobin and a solution depleted in hydrophobin, after which the surface carrying adsorbed hydrophobin is contacted with a solution containing a surfactant at a temperature lower than 90° C. to solubilize the hydrophobin adsorbed to said surface and separating a hydrophobin-enriched surfactant-comprising solution from said surface.
Method according to any of the preceding claims, characterized in that the hydrophobin is solubilized under a pressure of at least 1,1 Bar.
Method according to any of the preceding claims, characterized in that during adsorption of the hydrophobin at the surface, the pressure is reduced.
Method according to any of the preceding claims, characterized in that the surface has a contact angle for water larger than 60°.
Dependencies are inferred from the claim text.