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OwnerThomas Kolzau, Heinz Gerard Kohn +2inventors
InventorsThomas Kolzau, Heinz Gerard Kohn, Wilhelm Pluster, Mathias Ulbricht
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The invention relates to a method for purifying nucleic acids contained in a raw material using a porous membrane, the raw material possibly being prepurified and then mixed with a binding buffer containing a precipitant at a concentration such that the final concentration of the mixture of raw material and binding buffer it shall exceed the concentration required to precipitate nucleic acids, the raw material containing the nucleic acids being allowed to permeate the membrane in a manner that the nucleic acids shall be selectively retained at the membrane's surface or in its pores, the membrane being washed if called for and optionally the bound nucleic acids being eluted again from the membrane in a further step.
A method for purifying nucleic acids contained in a raw material, using a membrane, wherein
Method as claimed in one of the above claims, characterized in that the membrane is made of plastic.
Method as claimed in claim 5, characterized in that the membrane consists of polypropylenes, polyamides, polyesters, polysulfones, PVDF etc.
Method for purifying nucleic acids contained in a raw material, using a porous membrane functionalized with deprotonatable groups, wherein
Method as claimed in one of the above claims, characterized in that the membrane's pores are 0.2 to 10μ in diameter.
Method as claimed in one of the above claims, characterized in that the mebrane's thickness <500μ.
Method as claimed in one of the above claims, characterized in that the membrane is manufactured for use in micro-titration trays, spin columns or reaction containers.
Method as claimed in one of the above claims, characterized in that the binding-, washing- and/or elution-buffer is vacuum-aspirated through the membrane.
Dependencies are inferred from the claim text.